HPLC测定洋金花中东莨菪碱和阿托品的含量

Determination of scopolamine and atropine in Flos Daturae by RP-HPLC

目的:建立测定洋金花中东莨菪碱和阿托品含量的反相高效液相色谱方法。方法:采用Hypersil BDSC18柱(4.6 mm×250 mm,5μm),流动相甲醇-水(40∶60,水中含0.02 mol.L-1醋酸钠、0.02%三乙胺、用冰醋酸调pH至6.0),流速1.0 mL.min-1,检测波长215 nm,柱温室温。结果:东莨菪碱和阿托品的平均回收率分别为99.6%和100.4%;RSD为1.8%和1.5%。结论:该方法简便、准确,灵敏度高,重复性好。

Objective： To develop a quantitative analytical procedure of scopolamine and atropine in Flos Daturae using RP-HPLC. Method： The two alkaloids were separated on a Hypersil BDS C18 column （4.6 mm × 250 mm, 5 μm） with a mobile phase of 0.02 mol·L^- 1 sodium acetate buffer （containing 0.02% triethanolamine and the pH was adjusted to 6.0 with acetic acid）-methanol （60：40） and a detection wavelength of 215 nm. The flow rate was 1.0 mL·min^-1 and the column temperature was maintained at room temperature. Result： The mean recovery was （99.6 ± 1.8） % for scopolamine and （ 100.4 ±1.5 ） % for atropine. Conduslon： This method was simple, accurate and sensitive.