丹参多糖的提取分离及结构鉴定

Extraction,isolation and structure identification of polysaccharide in root of Salvia miltiorrhiza

目的:对中药丹参中多糖进行研究。方法:丹参药材经过水提醇沉得到丹参粗多糖,以DEAE-Sepharose Fast Flow纯化后,以H2O2脱色,流水透析,冷冻干燥后得到2个浅黄色均一多糖SMP 1,SMP 0.5,经13C-NMR,DEPT,IR谱,单糖组分分析、部分酸水解等方法鉴定其结构。结果:SMP 1,SMP 0.5相对分子质量分别约为1.39×106,4.03×105,SMP 1主要以-α(1→6)D-Glc聚合而成,有少量的-α(1→2)D-Glc聚合;SMP 0.5主要由-α(1→6)D-Glc聚合而成。结论:SMP 1,SMP 0.5为从丹参中首次分离得到的中性均一多糖。

Objective： To study the polysaccharide of Salvia miltiorrhiza. Method： The root was extracted by water and purified preliminarily by alcohol precipitation. The obtained crude polysaccharide purified by ion exchange resin DEAE-Sepharose Fast Flow then bleached with H2O2 and dialysed. Two portions of light yellow powder were obtained through lyophilization and named as SMP 1, SMP 0.5. On the basis of ^13C-NMR, DEPT, IR spectra, monesaecharide composite analysis and part monesaecharide composite analysis, their structures were identified. Result： The molecular weight of SMP 1, SMP 0.5 was estimated to be 1.39 × 10^6, 4.03 × 10^5, respectively, and the structure of SMP 1 was mainly α-（1→6） D-GIc linked a few α-（1→2） D-GIc, SMP0.5 was mainly a α-（1→6） D-Glucosan. Conclusion： SMP 1 and SMP0.5 were neutral homosaccharides that extracted from S. miltiorrhiza for the fast time.