摘要
目的:考察四物汤对骨髓基质细胞HFCL增殖及蛋白质表达的影响,探讨四物汤补血作用的分子机制。方法:分别用MTT法、流式细胞术和蛋白质组学方法测定四物汤对HFCL增殖、细胞周期及蛋白质表达的影响。结果:四物汤能促进HFCL的增殖,提高其增殖指数,并使HFCL的12种蛋白质表达上调,5种下调。结论:四物汤能促进HFCL增殖,作用于多个蛋白质靶点,间接发挥补血作用。
Objective： To investigate the effects of Siwu Tang on HFCL proliferation and protein expression of bone marrow stromal cells and to discuss molecular mechanism of enriching blood functions of Siwu Tang. Method： MTT, flow cytometry and proteomics were used respectively to measure the effect of Siwu Tang on HFCL proliferation, cell cycle and protein expression. Result： Siwu Tang can promote HFCL proliferation, increase proliferative index, up-regulate 12 kinds of proteins and down-regulate 5 kinds of proteins of HFCL. Conclusion： Through promoting HFCL proliferation and acting on multiple protein targets, the blood enriching function of Siwu Tang was exerted indirectly.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第13期1083-1086,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金资助项目(30271617)
全军医药卫生科研基金(01Z019)
北京市"248"重大创新工程项目(H010210220113)
关键词
四物汤
骨髓基质细胞
细胞增殖
蛋白质组学
Siwu Tang
bone marrow stromal cells
cell proliferaton
proteomics
