坤怡宁对雌性去卵巢大鼠生殖内分泌-免疫的影响

Effect of Kunyining capsule on reproductive ednocrine-immune functions in ovariectomized rats

目的:探讨坤怡宁颗粒治疗妇女更年期综合征的内在机制。方法:以去卵巢大鼠为模型,观察坤怡宁颗粒对去卵巢大鼠体重和子宫、胸腺、肾上腺等脏器系数的影响;用放免试剂盒检测血清雌二醇(E2),促卵泡激素(FSH),黄体生成素(LH),泌乳素(PRL),白细胞介素-2(IL-2),骨钙素(BGP)的含量。结果:坤怡宁能增加去卵巢大鼠子宫和肾上腺系数,提高其降低的E2,IL-2和BGP,显著降低去卵巢大鼠升高的FSH,LH和PRL。结论:坤怡宁对去卵巢大鼠神经内分泌-免疫紊乱有较好的调节作用。

Objective： To study the therapeutic mechanism of Kunyining（KYN） on the climacteric syndrome and provide the pharmacndynamie basis. Method： Ovariectomized rats were used as model to observe the effect of KYN on weight and the index of the organ of OVX rats. The concentration of E2, FSH, LH, PRL, BGP and IL-2 in serum were measured by competitive radio-immunological methods. Result： KYN could obviously increase the index of uterus and adrenal gland in OVX rats, and enhance the contents Of E2, IL-2 and BGP. The level of FSH, LH, PRL in serum were decreased. Conclusion： KYN can regulate beneficially the unbalanced reproductive endocrine-immune network, which is the pharmacological basis of KYN treating climacteric syndrome.