京尼平甙法测定酶活在β-葡萄糖苷酶高产菌株筛选中的应用

The application of lobster sauces deniposide enzyme activity to select the strain which has the high yield of β-glucosidase

在筛选β-葡萄糖苷酶高产菌株过程中用京尼平甙作底物来测定β-葡萄糖苷酶的活力,在对产物进行显色时,具有显色稳定、重现性好、试剂价格较低等优点。通过水杨苷法作葡萄糖标准曲线,能得到相对酶活与绝对酶活的换算公式,进而得到绝对酶活。实验采用京尼平甙作底物来测定β-葡萄糖苷酶的活力,即京尼平甙法,并从实验室保藏菌和豆豉分离菌中筛选出一株适合β-葡萄糖苷酶产量高的菌株O3。

During the selection of the strain which has the high yield of β- glucosidase the deniposide was used as the substrate to check the enzyme activity .The production which is colourated has some merits such as steady colouration, high repetition rate and low price of the reagent. Making standard curve by salicin enzyme activity ,we can get the formula of relative and absolute enzyme activity ,then transform into absolute enzyme activity of glucose. The method of using deniposide as the substrate laboratory was adopted to select the strain O3 which has the high yield of β-glucosidas, of which were chosen from the preserved enzyme and the losbster sauces enzyme.