我国南方媒介蜱中首次检出黑龙江立克次体(R.heilongjiangii)及马赛立克次体(R.massilliae)近缘菌

First identification of a rickettsia closely related to R.heilongjiangii and R.massilliae in South China

目的研究我国南方媒介蜱斑点热群立克次体带菌状况。方法采用斑点热群立克次体外膜蛋白ompA基因特异引物,对我国广东省连平县采集的长角血蜱进行检测分析。结果15组蜱标本中,14组检测阳性,阳性率为93.3%。通过测序分析,发现当地长角血蜱携带黑龙江立克次体及马赛立克次体近缘菌。结论我国南方地区除已证实的北亚蜱传斑点热外,还应加强当地其他斑点热的监测及临床鉴别诊断。

Objective To identify rickettsia carried by ticks collected from South China. Methods Primers derived from the ompA gene specific for spotted fever group rickettsia were used to amplify ompA gene from DNA samples extracted from ticks. Results A percentage of 93.3 of samples （14/15） were positive and firstly identified as a rickettsia closely related to R. heilongllangii and R. massilliae, Conclusion It is very important to survey and differentiate rickettsiae except for north Asian tick borne spotted fever in South China.