RFLP法检测PBMCs和血浆中HCV基因型的相关性与慢性化的关系

Determination of relation of HCV genotypes in plasma to those in PBMCs and relationship between HCV genotypes and hepatitis C chronicity by RFLP

目的检测HCV感染者血浆和外周血单个核细胞(PBMCs)中HCV基因型的相关性,同时研究不同型的HCV与丙型肝炎复发及慢性化的关系。方法应用特异性限制性片段长度多态分析(RFLP)—酶切分型法进行基因分型。结果82例血浆HCVRNA阳性的病例中1b(Ⅱ)型为37例(45.1%),2a(Ⅲ)型为34例(41.5%),1b/2a(Ⅱ/Ⅲ)型为11例(13.4%)。PBMCs中HCVRNA阳性的为54例(65.85%),其中1b型为38例,2a型为12例,1b/2a型为4例。结论HCV感染人体后,不但在血浆中可以检测到HCVRNA,而且也可以在PBMCs中检测到HCVRNA。同时HCV1b型比2a型更易感染PBMCs,HCV1b型感染者易出现慢性持续性感染,以至于发展为肝硬化。

Objective To study the relation of HCV genotypes in plasma to those in PBMCs of patients with hepatitis C and the relationship between HCV genotypes and relapse and chronicity of the disease. Methods Hepatitis C virus genomes were typed by restriction fragment length polymorphism （RFLP）. Results Fifty-four of the 82 anti-HCV positive and HCV RNA positive cases in plasma were HCV RNA positive in PBMCs. As for HCV genotype in plasma, it was type lb in 37 cases, type 2a in 34 and type lb/2a co-infection in 11. As for that in PBMCs, it was type lb in 38 cases, type 2a in 12, type lb/2a co-infection in 4. Conclusions HCV is not only present in plasma but also in PBMCs after infection, which indicates that there is extensive addiction of HCV RNA. PBMCs seems more likely to be infected by HCV of type lb than by that of type 2a （P〈0.01）. Meanwhile, type lb is more liable to chronic persistence of hepatopathy, and makes the patient＇s condition gradually worse and even develops into liver cirrhosis.