运动营养补剂对尾部悬吊大鼠比目鱼肌梭内、外肌纤维mATP酶活性的影响

Research on Effects of Cordyceps Sinensis in Activities of Myosin Adenosine Triphosphatase in Rats Soleus Muscle after Tailsuspension

目的:研究服用冬虫夏草对尾部悬吊大鼠比目鱼肌湿重及梭内、外肌纤维mATP酶活性的影响,旨在探讨在废用条件下冬虫夏草对梭内、外肌代谢的改变。方法:采用尾部悬吊法建立废用性肌肉萎缩模型,以钙增强的mATPase法检测废用条件下大鼠比目鱼肌梭内、外肌的mATP酶活性变化。结果:尾部悬吊可使梭外肌Ⅰ型肌纤维的构成比减少,Ⅱ型肌纤维的构成比增加,梭内肌纤维mATP酶活性增强,核袋1纤维mATP酶染色不变或由阴性转变为弱阳性,核袋2纤维和核链纤维由阳性转变为强阳性。服药组梭内、外肌mATP酶活性变化显著低于吊尾对照组(P<0.05),比目鱼肌湿重显著高于吊尾对照组(P<0.05)。结论:服用冬虫夏草对尾部悬吊大鼠比目鱼肌梭内、外肌纤维类型转化有明显的对抗作用。

Objective： To study the Effects of cordyceps sinensis in activities of myosin adenosine triphosphatase in rats soleus muscle after tailsuspension. Methods ： The activities of myosin adenosine triphosphatase of intrafusal and extrafusal fibers in soleus muscle were tested in 14d tail-suspended and then compared with control. Results ： The constituent ratios of type Ⅰ fiber decreased while that of type Ⅱ fiber increased. The activities of myosin adenosine triphosphatase of intrafusal fibers became higher after tail-suspension. Bag 1 intrafusal fibers showed medium activity instead of negative, while bag 2 intrafusal fibers and chain fibers showed higher activity when compared with control. The activity of myosin adenosine triphosphatase in control group was evidently higher activities than that in soleus muscle of drug-taking group（ P 〈 0.05）. Conclusion： The results showed that cordyceps sinensis had good effects in activities of myosin adenosine triphosphatase of intrafusal and extrafusal fibers in soleus muscle.