温度对3种产业化螺旋藻生长及蛋白质含量影响的研究

EFFECT OF TEMPERATUES ON GROWTH AND PROTEIN CONTENT OF THREE SPECIES OF SPIRULINA(ARTHROSPIRA)

本文研究了温度对内蒙古螺旋藻、钝顶螺旋藻和极大螺旋藻的生长和蛋白质含量的影响。结果表明，在每天光暗期各为12h，光照强度为180μmol·m^-2·s^-1条件下，3个藻种最适生长温度分别是：内蒙古螺旋藻为20℃～35℃，钝顶螺旋藻为20℃～30℃，极大螺旋藻为25℃～35℃；温度对这3个藻种蛋白质含量有不同的效应：在不同温度24h处理下，内蒙古螺旋藻在高温下积累较多的蛋白质，钝顶螺旋藻则在低温下积累较多的蛋白质，极大螺旋藻在最适温度下蛋白质含量最高；但较长时间的温度胁迫均使蛋白质含量降低，而内蒙古螺旋藻蛋白质含量降低幅度不显著；长时间的恒温不利于螺旋藻蛋白质的积累。内蒙古螺旋藻具有更好的抗高温和低温的能力，而且在较长时间的逆温胁迫下仍能保持较高的蛋白质含量，因此内蒙古螺旋藻具有更广阔的产业化生产前景。

The influence of temperature on growth and protein composition of three species of Spirulina, S. innermongoliansis, S. platensis and S. maxima used for food was studied. Under 12 - hour - light/12 - hour - dark photoperiod with photo flux density 180μnol m^-2·s^-1, optimal growth temperature is Verified for the three species： 20 to 35℃ for S. innermongoliansis, 20 to 30 ℃ for S. platensis and 25 to 35 ℃ for S. maxima. It is also observed that for the three species tested, the effect of temperature on protein content is significantly different. At the first 24 hours, S. innermongoliansis tends to accumulate more protein at higher temperature, while S. platensis is just opposite, and S. maxima accumulates more protein at its optimal temperature. But longer time of stress temperatures can cause significantly decrease of protein content, while the decrease of protein content of S. innermongoliansis is not so significant. Long - time constant temperature is not good for the accumulation of protein in Spirulina. S. innermongoliansis has better acclimation to high and low temperatures, and can maintain a relatively higher protein content at low and high temperatures than the other two species. So S. innermongoliansis has very large potential to be produced in mass culture.