摘要
在250mL摇瓶中研究了光自养、异养、混合营养3种营养模式对转兔防御素基因(NP-1)小球藻(Chlorella)的生长、NP-1表达量、总蛋白质含量、pH、葡萄糖消耗以及光衰减等几个方面的影响。结果表明,异养培养为转NP-1基因小球藻的最适营养模式。在此基础上,采用5,15,30L生物反应器补料分批异养培养转NP-1基因小球藻,细胞质量浓度分别达到3.67,4.87,6.39g/L。
The influences of culture modes including autotrophy, heterotrophy, mixotrophy on process characteristics of transgenic Chlorella with rabbit defensin （NP-1） gene in the 250mL flasks were investigated including cell growth, NP-1 expression, protein contents, pH, glucose consumption and light attenuation. The results showed that heterotrophy was the optimal culture mode for the transgenic Chlorella with NP- 1 gene. Based on these results, transgenic Chlorella was cultured in bioreactors of 5, 15, 30L and the corresponding cell densities were 3.67, 4.87, 6.39g/L, respectively.
出处
《海洋科学》
CAS
CSCD
北大核心
2006年第7期48-52,共5页
Marine Sciences
基金
国家863计划海洋生物技术主题资助项目(2002AA629110)
关键词
转基因小球藻
兔防御素
营养模式
表达
Transgenic Chlorella
rabbit defensin
culture modes
expression
