三种藻胆蛋白在复合累积LB膜中的能量传递

Study of Energy Transfer Among Three Kinds of Biliproteins in Their Stacking Langmuir-Blodgett Multilayers

制备了Ｒ－藻红蛋白（Ｒ－ＰＥ）、Ｃ－藻蓝蛋白（Ｃ－ＰＣ）及变藻蓝蛋白（ＡＰＣ）的单组分累积ＬＢ膜及三组分复合累积ＬＢ膜．吸收及荧光光谱测定表明三种蛋白的ＬＢ膜的光谱重叠性质与他们在水溶液中的性质相同．结构测定表明，三种蛋白在其ＬＢ膜中排列有序，且由它们组装的复合累积ＬＢ膜结构类似于藻类植物中的藻胆体结构．通过稳态荧光光谱及其光谱解叠，观察到了三种蛋白在复合累积ＬＢ膜中的激发能量传递现象．根据给体荧光峰的猝灭，计算出在复合累积ＬＢ膜中从给体Ｒ－ＰＥ经Ｃ－ＰＣ到受体ＡＰＣ的能量传递效率为５１％．

Single comPOnents and multicomponent of R-phycoerythrin (R-PE ), Cphycocyanin (C-PC ) and allophycocyanin(APC) LB multilayers were prepared. The threephycobiliproteins in the LB films were orderlyoriented as in the natural phycobilisomes. Theabsorption and fluorescence spectra remainedsimilarly to these in their aqueous solutions. Thephenomenon of excitation energy transfer amongR-PE, C-PC and APC in their stacking LB multilayers were observed through steady-state fluorescence spectral measurements and spectraldeconvolutions. From the quenching degree ofR-PE fluorescence, the efficiency of energytransfer from R-PE through C-PC to APC intheir stacking LB multilayers was calculated tobe 51 %.