鼠肝抽提液中磷酸酶活性终止方法的改进

Improvement of Termination in the Phosphatase Activity Assay of Crude Liver Extract From Mice.

改进了一种分析磷酸酶活性的终止酶反应方法该方法通过在酶反应进行到一定程度时，在反应混合物中加入酶反应终止液（１ｍｏｌ／ＬＮａＯＨ－０．２ｍｏｌ／ＬＥＤＴＡ），从而使测定更简捷。

A phosphatase activity assay wherep-nitrophenyl phosphate was used as substratewas improved by use of the terminating reagentcontaining 1 mol/L NaOH-0.2 mol/L EDTA.Abetter assay result was obtained.