RP-HPLC法测定清肺抑火片的含量

Determination of Three Components in Qingfei Yihuo Tablets by RP-HPLC

建立清肺抑火片含量测定的RP-HPLC法。选用Agilent Eclipse XDB C18色谱柱，甲醇-乙腈-1．5％醋酸溶液为流动相作梯度洗脱；检测波长280nm。黄芩苷在0．12～3．60μg的范围内呈良好的线性关系，r=0．9999，平均回收率为97．58％（n=5），RSD=1．71％；大黄素在0．012～0．360μg的范围内呈良好的线性关系，r=0．9999，平均回收率为97．86％（n=5），RSD=1．81％；大黄酚在0．02～0．60μg的范围内呈良好的线性关系，r=0．9999，平均回收率为98．65％（n=5），RSD=1．88％。本法操作简便准确，测定清肺抑火片的含量，便于全面控制该制剂的质量。

An RP-HPLC method was established for determination of three components in Qingfei Yihuo tablets. An Agilent Eclipse XDB C18 column was used with methanol-acetonitrile-1.5% acetic acids as mobile phase for gradient elution. The detection wavelength was 280 nm. There was a good linear relationship when Baicalin was 0. 12 -0.60μg （r=0. 9999）, the average recovery was 97.58% （n= 5）, and R SD= 1.71%. The good linear relationship for Emodin and Chrysophanol were 0. 012-0. 360μg （r= 0. 9999） and 0.02-0. 60μg （r=0. 9999） respectively, and the average recovery were 97.86% （n=5, RSD =1.81%） and 98. 65% （n=5, RSD=1. 88%） accordingly. The method was simple and accurate. It could be used for quality control of Qingfei Yihuo tablets.