大白菜小孢子培养影响因素研究

Studies on the Effective Factors of Microspore Culture in Chinese Cabbage

以福田50、长快两个大白菜一代杂种为试材,对影响小孢子胚状体形成的几个因素进行了研究.结果表明,0.1～1.0 mg·L^-1的NAA对大白菜小孢子胚的发生有抑制作用,而且随生长素浓度增高抑制作用加大.2,4-D对小孢子胚的形成有强烈的抑制作用.较低浓度的细胞分裂素能促进胚状体的发生,而较高浓度则产生抑制作用,ZT的最适宜浓度为0.2 mg·L^-1,6-BA的最适浓度为0.4 mg·L^-1,且6-BA的作用大于ZT.生长素和细胞分裂素的综合影响是两者影响的叠加.NLN培养基是最适于大白菜游离小孢子培养的基本培养基.活性炭对大白菜小孢子胚的发生有抑制作用.

The experiment on culture technique of the isolated micraspore was conducted with Futian 50 and Changkuai genotypes of Chinese cabbage as the materials. The results showed that 0.1 - 1.0 mg ·L^-1 NAA inhibited the embryo emergence. 2,4 - D strongly inhibited the formation of mierospore embryo. Low density of etytokinin improved the frequency of mierospore embryogenesis. The optimal content of ZT was 0.2 mg·L^-1 and 6 - BA was 0.4 mg· L^-1. The influence of 6 - BA is greater than ZT. Comprehensive influence of auxin and etytokinin was the superposition of the both influences. NLN culture medium is optimal for the isolated mierospore of Chinese cabbage euhure. The active ehureoal of 100,200 mg· L^-1 did not promote the mierospore embryogenesis.