电针足三里穴对LPS刺激后大鼠胃排空功能和延髓迷走孤束复合体内的神经元Fos表达的影响(英文)

Effects of electro-acupuncture on gastric emptying and Fos expression in the neuron of vagal-solitary complex of rat bulbus after LPS stimulation

目的观察电针足三里穴干预后腹腔注射细菌内毒素(LPS)大鼠的胃排空功能和延髓迷走孤束复合体内神经元Fos的表达变化及其意义。方法雄性SD大鼠40只,随机分为空白对照组、LPS组、LPS后电针足三里穴组和LPS后电针非经非穴组,每组10只。采用免疫组织化学方法,观察并计数大鼠延髓迷走孤束复合体内神经元Fos的表达并计算各组动物的胃排空率。结果腹腔注射细菌内毒素(LPS) 2·5h后大鼠的胃排空率明显降低至20·7 %±4·5 %,迷走孤束复合体内Fos阳性神经元数目明显增加至83·2±6·6 ;LPS后电针足三里穴组大鼠的胃排空率明显升高至44·1 %±6·2 %,迷走孤束复合体内Fos阳性神经元数目明显减少至37·9±3·8 ,与LPS组比较有显著性差异(P<0·01) ;LPS后电针非经非穴组的胃排空率和迷走孤束复合体内Fos阳性神经元数目与LPS组无明显差异。结论腹腔注射LPS对大鼠胃排空功能有明显的抑制作用;电针足三里穴对LPS大鼠的胃排空功能具有良性的调节作用,此作用与其保护性调节延髓迷走孤束复合体内的神经元功能状态有关。

Objective To investigate the effects of electro-acupuncture on gastric emptying and Fos expression in the neuron of vagal-solitary complex （VSC） of rat bulbus after lipopolysaeeharide （LPS） intraperitoneal injection （i. p. ）. Methods 40 male SD rats were randomly assigned to 4 groups： control group, LPS i.p. group, LPS i.p. plus electro-acupuncture at Tsusanli point group and LPS i.p. plus electro-acupuncture at non-meridian-non-acupoint group, 10 rats for each group. Immunohistocbemical techniques were used to detect the Fos expression in VSC. The animal＇s gastric emptying was measured by phenol red method. Results The rats with gastric emptying decreased greatly to （20. 7±4. 5）% 2. 5 hours after LPS injection, and Fos-positive neurons were significantly found in VSC （83. 2±6. 6） compared with control group. However, in the group of LPS i. p. plus electro-acupuncture at Tsusanli point, the gastric emptying was up-regulated to （44. 1±6. 2）%, and the expression of Fos-positive neurons were down-regulated to （37. 9±3. 8） compared with LPS i. p. group. No significant difference was found between the group of LPS i.p. plus electro-acupuncture at norrmeridian-non-acupoint and the group of LPS i. p. Condmion LPS i. p. can retard the gastric motility in rats, electro-acupuncture at Tsusanli point may well regulate the function in LPS model rats. This function may be connected with its protective effects on Fos immunoreactive neurons activity in VSC of rat bulbus.