摘要
By Using the modified method to extract Rice dwarf virus virions from infected rice leaves,a concentration 3.854mg/mL of purified virus virions reached,and the Rabbits was immunized with purified virions,yielding high titer antiserum;1:20480 by the indirect enzyme-linked immunosorbent assay(I-ELISA)test.The antiserum successfully detected three rice samples from Kunming,Yuxi and Luliang by I-ELISA.Immunocapture polymerase chain reaction(IC-PCR),respectively was the utilized to show that serologic and molecular methods were successfully used for detection of Rice dwarf virus.
By Using the modified method to extract Rice dwarf virus virions from infected rice leaves, a concentration 3.854mg/mL of purified virus virions reached, and the Rabbits was immunized with purified virions, yielding high titer antiserum; 1：20480 by the indirect enzyme-linked immunosorbent assay （I-ELISA） test. The antiserum successfully detected three rice samples from Kunming, Yuxi and Luliang by I-ELISA.Immunocapture polymerase chain reaction （IC-PCR）, respectively was the utilized to show that serologic and molecular methods were successfully used for detection of Rice dwarf virus.
出处
《中国病毒学》
CSCD
2006年第4期408-410,共3页
Virologica Sinica
基金
国家重点基础研究发展规划项目(G2000016201)
