葡萄蔗糖转运蛋白在烟草中的反义表达及其对转基因烟草的影响

The Antisence Expression of a Putative Grape Sucrose Transporter in Tobacco and Effecs on Growth of The Transformants

将葡萄蔗糖转运蛋白VvSUC27cDNA以反义方向插入到含有CaMV35s启动子的真核表达载体pBI121载体中,然后转化到烟草(Nicotianatobacumcv.Samsun)植株中.转反义VvSUC27cDNA的烟草植株在含有20g/L蔗糖的培养基上能够正常生长发育,但是通过切片观察,发现其根部发育较弱,且叶片叶绿体含量增加.可溶性糖测定发现,转基因烟草根部蔗糖含量只有野生型烟草的51%.14C蔗糖吸收实验发现转基因烟草转运外界蔗糖的能力大大降低.

A cDNA （VvSUC27） of grape sucrose transporter was inserted into an expression vector pBI121 in the anti-sense orientation under the control of the cauliflower mosaic virus 35 S promoter, and subsequently transferred into tobacco（Nicotiana tobacum cv. Samsun）. The plantlets of the transformed anti-sense VvSUC27 cDNA tobacco were grown normally on MS media containing 20g/L sucrose, but it was found that roots of the transformants developed slowly and the leaves have more chloroplasts when observed under slice. Sucrose content in the roots of the transformants was only 51% of the controls. The capability of sucrose uptaking and transporting into leaves among the transformed tobaccos was greatly decreased.