日本血吸虫不同方式感染宿主的免疫病理研究

Immunopathological observations on the infection of Schistosoma japonicum induced by different manners

目的建立等量血吸虫尾蚴单次感染和多次感染的动物模型,比较这两种不同感染方式对家兔肝脏的损害程度。方法制作肝脏病理切片,测量单个虫卵肉芽肿的体积;放免法检测家兔血清中透明质酸(HA)、层粘连蛋白(LN);RT-PCR-ELISA半定量测定肝组织中的TGF-β1mRNA。结果单次感染组家兔急性期肉芽肿反应最明显,慢性期和晚期肉芽肿体积逐渐缩小,而多次感染组肉芽肿缩小不明显;血清中HA和LN、肝组织中的TGF-β1mRNA量,在感染各组均高于正常对照,但多次感染组升高更明显。结论等量的尾蚴,多次感染比单次感染的危害引起的肝纤维化更为严重。

The liver damages in rabbits induced by single and multiple infection with same number of Schistosoma japonicum cercariae were compared in the present investigation, in which the size of single-egg granuloma was measured in the pathologieal sections of liver, hyaluronic acid （HA） and laminin （LN） in serum were tested with RIA and TFG-β1 mRNA in liver tissues was determined by RT-PCR-ELISA assay. In case of single infection it was demonstrated that the granuloma response in liver appeared to be rather prominent in acute stage with large size of egg granuloma, but they became smaller during chronic or latter stage. However, in case of multiple infection, the shrinking of granuloma was not so prominent. The contents of HA and LN in sera and TGF-β1 mRNA in liver tissues were all higher than those of the normal controls, but the elevation was even more prominent in case of multiple infection. It is concluded that the multiple infection with same numbers of cercariae can elicit greater damages to the host than that of the single infection.