水稻白叶枯病成株抗性相关基因的表达谱

Expression Profiling of Genes Related to Adult Plant Resistance to Bacterial Blight of Rice

采用cDNA-AFLP(amplifiedfragmentlengthpolymorphism)分析了水稻白叶枯病成株抗性相关基因的表达。在检测的16000个基因片段中,122个表现为差异表达。对其中的70个片段进行了克隆和测序,有41个片段编码的氨基酸序列与已知基因同源。通过Northern杂交和RT-PCR分析了29个功能已知基因的表达,检测到10个基因表现出表达差异。这些基因编码蛋白参与电子和质子转运、泛素—蛋白体途径以及表观遗传调控(epigeneticregulation)等。这些基因在不同处理的水稻苗期和成株期叶片中存在表达差异,表明它们可能在成株抗性中发挥重要作用。

Genes related to adult plant resistance （APR） of rice to Xanthomonas oryzae pv. oryzae were detected by using cDNA-AFLP （amplified fragment length polymorphism）. Of 16 000 fragments inspected, 122 showed differential expression patterns. 70 fragments were cloned and sequenced. 41 fragments had amino acid sequences homologous to known proteins. Northern blots and RT-PCR analysis were used to test the expression of 29 fragments which encoded proteins with identified function and of these, 10 fragments were verified to be associated with APR. These fragments encode proteins involved in electron transfer and proton transport, the ubiquitin-proteasome pathway, and epigenetic gene regulation. Differential expression of these fragments between seedlings and adult plants suggests that they may play a pivotal role in rice APR to bacterial blight.