稻瘟病菌激发子诱导的水稻叶片mRNA差异表达研究

mRNA Differential Exppression of Rice Induced by Elicitor from Magnaporthe grisea

本文主要以广州优势水稻稻瘟病菌小种ZC13(97-151a)和以CO39为背景的水稻近等基因系C101LAC为材料,研究水稻激发子与稻瘟病菌之间的互作关系。采用mRNA差异显示技术,将得到的可能差异条带进行重扩增和纯化,最后得到差异显著的条带,采用重复试验,对重复性好且稳定的部分条带利用pMD18-T载体及大肠杆菌DH5α进行克隆测序,NCBI比对分析,发现某些差异片段与水稻的激酶及锌指蛋白有较高的同源性。

In order to reveal the molecular mechanisms of interaction between elicitor and rice, experiments were conducted with C 101LAC, the near isogenic lines of rice sharing CO39 background inoculated with M. grisea and one strain 97-151a of race ZC13, one of the prevalent races office blast fimgus M. grisea in Guangdong province. Differential cDNA fragments were performed by differential display polymerase chain reaction （DD-PCR） technology. Then the differential cDNA fragments were recovered from the gels and reamplified and purified. These fragments were transformed to pMD18-T vector and E. coli DH5a and sequenced and analyzed in NCBI. It indicated zinc finger protein and receptor protein kinase possibly participate in rice disease resistance to rice blast.