三七植物GAPDH基因克隆及序列分析

Cloning and Sequencing of Panax notoginseng GAPDH

采用改进的异硫氰酸胍法提取高质量三七总RNA,运用RT-PCR方法克隆了三七GAPDH基因的部分序列,长度为627 bp,编码209个氨基酸,为半定量RT-PCR以及Real-time RT-PCR等技术在三七植物研究中的应用提供了条件.氨基酸序列比对结果表明,该序列与拟南芥、烟草、人参的GAPDH氨基酸序列的同源性分别为91%、93%、95%;核苷酸序列的同源性分别为82%、84%、85%.

The total RNA of Panax notoginseng was extracted by modified guanidine thiocyanate method and the partial sequence of P.notoginsengGAPDH was cloned by RT-PCR,which was 627 bp long and encoded 209 amino acids. This partial sequence provided the condition to apply RT-PCR and Real time RT-PCR to P. notoginseng research. It was 91% ,93% and 95% homologous to the sequences of GAPDH amino acids in Arabidopsis thaliana ,tobacco and Panax ginseng and 82%,84% and 85% homologous to the sequences of GAPDH nucleotides in these plants,respectively.