大鼠精索静脉曲张后附睾上皮细胞凋亡及管腔α-1,4-葡糖苷酶、唾液酸含量观察

Effects of Experimental Varicocele on the Apoptosis of Epididymis Epithelium and Synthesizing Function of the Epididymis in Rats

目的:探讨大鼠左侧精索静脉曲张后同侧附睾上皮细胞凋亡与附睾管腔中-α1,4-葡糖苷酶和唾液酸含量改变的关系及意义。方法:16只雄性SD大鼠建立左侧精索静脉曲张模型,分为对照组和实验组,每组8只。缺口末端转移酶标记技术检测附睾上皮细胞凋亡;硝基酚法和5-甲基苯二酚法分别检测附睾管腔液-α1,4-葡糖苷酶和唾液酸含量。结果:大鼠左侧精索静脉曲张模型建立后的第7 d,实验组同侧附睾上皮细胞凋亡指数显著高于对照组(P<0.001);实验组附睾管腔液-α1,4-葡糖苷酶、唾液酸含量显著低于对照组(P<0.05,P<0.005)。结论:大鼠左侧精索静脉曲张可导致同侧附睾上皮细胞凋亡增加,进而影响该侧附睾上皮细胞的合成和分泌功能。

Objective： To study the relationships of experimental varicocele to the apoptosis of epididymis epithelium and changes of the contents of α-1,4-glucosidase and sialic acid from the unilateral epididymis in adolescent rats, and to investigate the effects of varicocele on the unilateral epididymis epithelium. Methods： Experimental left varicocele models of 16 adult male Sprague-Dawley rats were obtained by partial ligation of the left renal vein. The epididymides were collected for detecting the apoptosis of epididymis epithelium and the contents of α-1,4-glucosidase and sialic acid by using spectrophotometry. Results ： Seven days after the establishment of the left varicocele model, the index of the apoptosis of the left epididymis epithelium was significantly higher （ P 〈 0. 001 ） and the contents of α-1,4-glucosidase and sialic acid significantly lower （ P 〈 0.05, P 〈 0. 005 ） in the experimental group than in the control.