趋化因子受体CXCR4／CXCL12信号转导通路在结直肠癌肝转移中的作用

Role of chemokine receptor CXCR4/CXCL12 signaling pathway in hepatic metastasis of colorectal carcinoma

目的:探讨CXCR4／CXCL12在结直肠癌肝转移中的作用．方法:应用Western blot检测160例结直肠癌患者标本中肿瘤组织、邻近正常黏膜以及肝转移组织中CXCR4／CXCL12通路成员的表达情况,免疫组织化学法检测CXCR4／CXCL12在细胞水平的分布．结果:与正常组织相比,结直肠癌组织中 CXCR4／CXCL12表达水平明显增高(P<0．05); 与原发肿瘤相比,10例肝转移组织中CXCR4／ CXCL12表达增高(CXCR4:3．9±0．5 vs 2．2± 0．3,P<0．05:CXCL12:3．6±0．5 vs 2．4±0．3, P<0．05):TNMⅢ、Ⅳ分期CXCR4／CXCL12表达水平比Ⅰ、Ⅱ分期显著增加有关(CXCR4: 3．4±0．6 vs 1．8±0．3．P<0．05;CXCL12:3．6± 0．5 vs 1．8±0．4．P<0．05)．结论:趋化因子受体CXCR4／CXCL12在原发结直肠癌与肝转移组织中呈高表达,CXCR4／ CXCL12信号转导通路可能在结直肠癌肝转移过程中起一定作用．

AIM： To investigate the potential role ot chemokine receptor CXCR4/CXCL12 signal transduction pathway in hepatic metastasis of colorectal carcinoma. METHODS： CXCR4 and CXCL12 expression were detected in tissue samples from 60 patients with colorectal carcinoma representing various clinicopathological parameters by Western blot analysis. RESULTS： The levels of CXCR4 and CXCL12 proteins expression were increased significantly in colorectal cancer as compared with those in the adjacent normal mucosa （P〈0.05）. In the 10 patients with lymph node metastasis, the expres-sion of CXCR4 and CXCL12 were also markedly higher than those in those without metastasis （CXCR4：3.9±0.5 vs 2.2±0.3, P〈0.05; CXCL12：3.6±0.5 vs 2.4±0.3, P 〈0.05）. The over-expression of CXCR4 and CXCL12 proteins were correlated with TNM staging, and they were highly elevated in colorectal cancer atⅢ andⅣ stages as compared with those at I and Ⅱ stages （CXCR4：3.4±0.6 vs 1.8±0.3, P〈0.05; CXCL12： 3.6± 0.5 vs 1.8±0.4, P〈0.05）. CONCLUSION： Over-expression of CXCR4/ CXCL12 plays an important role in the hepatic metastasis of colorectal carcinoma.