幼鼠肠缺血／再灌注损伤肠组织TNF-α和c-fos mRNA的表达

Expression of tumor necrosis factor-α and c-fos mRNA in immature rats' model of gut ischemia/reperfusion injury

目的:观察幼鼠肠缺血／再灌注损伤(I／R)不同时点肠组织内肿瘤坏死因子α(TNF-α)、c-fos mRNA表达模式,探讨肠I／R的机制．方法:分离幼鼠肠系膜上动脉,制作动物模型,应用RT-PCR半定量法分析肠组织 TNF-α、c-fos mRNA表达．结果:与假手术组相比,肠组织内TNF-α mRNA缺血30 min显著升高(1．55±0．33 vs 1．07±0．08,P<0．05),再灌注30 min达峰值(3．05 ±0．11),再灌注后60,90 min逐渐下降;c-fos mRNA表达于缺血30 min后升高(0．95±0．13 vs 0．12±0．02,P<0．05),再灌注30 min达峰值 (1．53±0．11),再灌注后60 min迅速下降,再灌注后90 min达基线水平．结论:幼鼠肠I／R损伤介导肠组织内TNF-α和 c-fos mRNA的表达模式．c-fos mRNA表达伴随TNF-α mRNA表达,提示二者间可能存在关系．

AIM： To analyze the expression patterns of tumor necrosis factor-or （TNF-α and c-fos mRNA in immature rats after gut ischemia/reperfusion （I/ R） injury. METHODS： The left laparotomy was performed and the superior mesenteric artery （SMA） was occluded by artery clamp to establish the model of gut I/R injury. The expression of TNF-α and c-fos mRNA were detected by reverse transcription polymerase chain reaction （RT-PCR）. RESULTS： In comparison with sham operation group, an increase in TNF-α mRNA was detected after 30-min ischemia （1.55±0.33 vs 1.07 ±0.08, P〈0.05） and the maximal increase was detected after 30-min reperfusion （3.05±0.11）. TNF-α mRNA expression after 60-min reperfusion （2.02±0.10） remained above the control level. The expression of c-fos mRNA in the intestinal tissues was rapidly induced by ischemia, and it increased obviously 30 min after ischemia as compared with that in sham operation group （0.95± 0.13 vs 0.12±0.02, P〈0.05）. The expression of c-fos mRNA also reached the peak level 30 min after reperfusion （1.53± 0.11）, and it declined markedly 60 min after reperfusion. After 90-min reperfusion, c-fos mRNA expression returned to the normal level. CONCLUSION： Gut I/R induces the mRNA expression of both TNF-α and c-fos, suggesting that there is possibly a correlation between the expression of c-los and TNF-α.