G蛋白β_3基因多态性同维吾尔族人群高血压的关联分析

Association study on GNB_3 gene polymorphism with essential hypertension in Xinjiang Uiygur group

目的研究G蛋白β3亚单位(G-proteinβ3Subunit,GNB3)基因第10外显子C825T多态性同中国维吾尔族人群高血压之间的关联性。方法采用较大样本巢式病例-对照研究(n=738),用聚合酶链式反应(PCR)及限制性片段长度多态性(RFLP)技术对354例高血压患者及384例正常血压者GNB3基因C825T多态进行检测。结果GNB3基因C825T多态3种基因型CC、TT、CT分布频率在病例组为27.2%、42.9%、29.9%,在正常对照组为27.7%、42.4%、29.9%,基因型分布在组间的差异无统计学意义(2χ=0.026 2,P=0.99)。T等位基因分布频率在病例组为51.4%、在正常对照组为51.2%,组间的差异也无统计学意义(χ2=0.001 6,P=0.97)。对病例组在不同基因型间进行比较,未发现CC、CT、TT基因型间收缩压、舒张压、体重指数、血糖、血脂水平有统计学差异。结论GNB3C825CT多态可能与维吾尔族人群高血压无关联。

Purpose To investigate the relationship between the 10^th exon C825T of G-protein β3 subunit（GNB3） genetic polymorphism and hypertension in Uygur population of China. Methods A nested case-control study（n = 738） was carried out. Polymerase chain reaction and restrict fragment length polymorphism（PCR-PFLP） technique was used to detect the genotype GNB3 C825T polymophism in 354 hypertensive and 384 normaltensive （NT） Uiygur subjects. Results The distributions of GNB3 C825T genotypes were CC （27.2%）, TT （42.9%）, CT（29.9%） in the hypertensive subjects and CC （27.7%）, TT （42.4%）, CT （29.9%） in the normotensive subjects. There were no significant differences of both genotype distribution between the two groups （x^2 = 0. 026 2, P = 0.99）. The T allele was 51.4% in hypertensive subjects and 51.2% in normotensive subjects, which were no significant differences between the two groups （x^2 = 0. 0016 P = 0.97）. Further analyses showed that there was no association between C825T genotypes and Age, BMI, Glu, Triglyceride, Cholesterol, systolic blood pressure （BP） and diastolic BP. Conclusions No evidence was found to suggest an association of the GNB3 C825T polymorphism with hypertension in the Uygur population of China.