RP-HPLC法测定柘树根中四种异戊烯基口山酮的含量

Determination of four prenylated xanthones in the roots of Cudrania tricuspidata by RP-HPLC

目的建立高效液相色谱法（HPLC）测定柘树根中4种异戊烯基咄酮的含量。方法采用Hyper—Clone BDS C18（4．6mm×250mm，5μm）色谱柱，以甲醇-乙腈-0．2％磷酸为流动相，梯度洗脱，流速0．8mL／min，检测波长259nm，柱温50℃。结果4种异戊烯基[口山]酮在一定范围内均呈良好线性关系，平均加样回收率在97.03％～103．25％范围内，RSD〈3．86％（n=6）。结论本方法结果准确、重复性好。

Purpose To develop a RP-HPLC method for determination of four prenylated in the roots of Cudrania tricuspidata（Carr. ）Bur. Methods The HPLC separation was xanthones carried out on a HyperClone BDS C18 （4.6 mm × 250 mm, 5μm） column, using methanol-acetonitrile-0.2 % H3PO4 as the mobile phase for gradient elution. The flow rate was 0.8 mL/min,the detection wavelength was 259 nm,and the temperature of column was 50℃. Results Linearity was confirmed for the four prenylated xanthones. The average recoveries were in the range of 97.03 %-103.25 % with RSDs less than 3.86% （n = 6）. Conclusions The method is accurate, repeatable and applicable to the determination of four prenylated xanthones in C. tricuspidata.