T细胞激活剂对Jurkat细胞重组激活基因表达的影响

Effects of T cell activators on mRNA expression of recombination-activating genes in Jurkat cells

目的:研究T细胞激活剂PHA,抗CD3mAb,PMA+A23187对人T细胞白血病细胞株Jurkat重组激活基因(RAGs)mRNA表达水平的影响.方法:采用RTPCR法检测不同T细胞激活剂作用不同时间后Jurkat细胞中RAG1,RAG2mRNA,以β肌动蛋白(βactin)的表达作为内参照进行灰度分析,比较不同组Jurkat细胞RAGsmRNA的表达水平.结果:三种T细胞激活剂刺激诱导的Jurkat细胞RAG1mRNA表达量明显低于对照组(P<0.05),且RAG1mRNA表达量的下降与刺激诱导作用时间有关;PHA刺激诱导后RAG2表达量显著低于对照组(P<0.05);PMA+A23187刺激诱导6,12h均未检测出RAG2mRNA的表达;而抗CD3mAb作用12h组RAG2mRNA表达量显著高于对照组(P<0.05).结论:Jurkat细胞同时表达RAG1,RAG2mRNA,并在一定诱导下RAGs表达量发生改变,因此有可能成为研究外周T细胞RAGs表达调节的一个潜在的细胞模型.

AIM： To investigate the effects of T cell activators on the mRNA expression of recombination-activating genes （RAGs） in Jurkat cells. METHODS： After Jurkat cells were treated with PHA （ 20 mg/L）, anti-CD3 mAb （ 1 ： 100 ）, PMA （40μg/L） ＋ A23187 （0.5 μmol/L） for6 and 12 h respectively, RAG-1 and RAG-2 mRNA were measured by RT-PCR. Semiquantitative analysis was used to evaluate RAG-1 and RAG-2 mRNA levels in different groups. REULTS： Compared with the control groups, the levels of RAG-1 mRNA were significantly lower in different treated groups （ P 〈 0.05 ）, and RAG-1 mRNA levels were associated with treatment time. RAG-2 mRNA levels in PHA treated groups were significantly lower than those in the control groups （ P 〈 0.05）. No RAG-2 mRNA was detected in PMA ＋ A23187 treated groups. However, RAG-2 mRNA level was significantly higher in anti-CD3 mAb （ 12 h） treated group than that in control groups（ P 〈 0.05 ）. CONCLUSION： RAG-1 and RAG-2 mRNA were coexpressed in Jurkat cells and could be regulated by T cell activators. The results may give a clue that Jurkat cells maybe provide an ideal cell line model for studying the regulation of RAGs in peripheral T cells.