食管鳞癌中TGF-β及其受体调节成分Endoglin/CD105的表达

Overexpressions of TGF-β and its receptor regulatory component Endoglin/CD105 in esophageal squamous cell cancer

目的:研究食管鳞癌中TGFβ和Endoglin/CD105的表达及在血管生成中的作用.方法:采用RNA酶保护性分析和免疫组织化学方法对30例食管癌高发区食管癌患者的癌组织和癌旁组织TGFβ和Endoglin/CD105mRNA和蛋白表达水平进行检测.结果:食管癌组织中TGFβ1和TGFβ3mRNA较癌旁组织显著增高(P<0.05),TGFβ2无变化.食管癌组织中Endoglin/CD105mRNA较癌旁组织也显著升高(P<0.05).TGFβ3mRNA与Endoglin/CD105mRNA的相关分析显示有相关关系.结论:TGFβ和Endoglin/CD105参与了食管鳞癌血管生成过程的调节,Endoglin/CD105不仅是细胞膜上TGFβ受体中调节TGFβ生物学功能的一个重要分子,而且是新生血管的重要标志.因此调节Endoglin/CD105的功能可能成为抗血管治疗中的一个潜在的、具有较宽生物防治谱的靶点.

AIM： To investigate the expressions of TGF-β and Endoglin/CD105 in esophageal carcinoma from patients in high incidence area in north China. METHODS： The expressions of TGF-βand Endoglin/CD105 mRNA and protein were detected in 30 esophageal carcinoma samples using RNase protection assay and immunohistochemical staining. RESULTS： TGF-β1, TGF-β3 and Endoglin/CD105 mRNA, other than TGF-β2, were significantly increased in esophageal carcinoma than those of adjacent noncancerous tissue（ P 〈 0.05 ）. There was a significant correlation between TGF-133 and Endoglin/CD105 at mRNA level. CONCLUSION： The overexpressions of TGF-β1, TGF-β3 and Endoglin/CD105 may be involved in the regulation of angiogenesis in esophageal carcinoma. Eudoglin/CD105 is an important anglogenesis marker as well as regulatory component on cell membrane in regulating the biological function of TGF-β. Endoglin/CD105 could be a potential target to anti-angiogenesis therapy.