二乔玉兰花粉贮存条件的比较研究

Preservation Methods of Pollen of Magnolia soulangeana Soul.-Bod.

探讨了二乔玉兰(MagnoliasoulangeanaSoul.-Bod.)花粉在不同温度和贮存条件下的活力。结果表明:二乔玉兰花粉在5%蔗糖+0.01%硼酸及5%蔗糖+0.1%硼酸两种培养液上萌发较好,萌发率均达到70%以上。随着保存时间的延长,花粉萌发率不断降低,降低速度从快到慢依次为室温、5℃和-20℃。超低温(-196℃)保存花粉的萌发率并没有随着保存时间的延长而降低,液氮保存2a的花粉萌发率达到79.3%,与新鲜花粉的萌发率差异不显著。超低温反复冻存6次的花粉萌发率与新鲜花粉没有显著变化。

Viability of pollen of Magnolia soulangeana was tested at different storage temperatures and methods. Culture solution of 5% sucrose supplemented with 0.01% or 0.1% boric acid gave good results for pollen germination, the germination rate reaching to above 70%. Pollen viability declined with the increased preservation time. The decrease of pollen viability was slowered in the order of room temperature, 5℃, and -20℃. Cryogenic storage at -196℃ had no significant changes, even though the pollen stored for 2 years, the germination rate being 79.3%. Pollen samples exhibited high tolerance to re-freezing and thawing.