摘要
The capsid protein encoded by ORF2 of human calicivirus(HuCV) expressed in the baculovirus expression system can self-assembled into virus-like particles(VLPs),which would be important for the development of improving diagnostic methods.The RT-PCR procedures routinely used to amplify the RNA polymerase region have usually been inefficient for amplification of ORF2,which would bring obstacle for the development of new diagnostic reagents.Through optimization of RT-PCR conditions,we established a simple and high performance RT-PCR method to amplify ORF2,and by which demonstrated a corresponding relationship between the genotype and the antigenic type.It provides conditions for developing diagnostic reagent which accommodates to diagnosis of HuCVs prevailing in China,and also provides the basis for studying the genetic variance and the pathogenesis of HuCV.
The capsid protein encoded by ORF2 of human calicivirus （HuCV） expressed in the baculovirus expression system can self-assembled into virus-like particles（VLPs）, which would be important for the development of improving diagnostic methods. The RT-PCR procedures routinely used to amplify the RNA polymerase region have usually been inefficient for amplification of ORF2, which would bring obstacle for the development of new diagnostic reagents. Through optimization of RT-PCR conditions, we established a simple and high performance RT-PCR method to amplify ORF2,and by which demonstrated a corresponding relationship between the genotype and the antigenic type. It provides conditions for developing diagnostic reagent which accommodates to diagnosis of HuCVs prevailing in China, and also provides the basis for studying the genetic variance and the pathogenesis of HuCV.
出处
《病毒学报》
CAS
CSCD
北大核心
2006年第4期320-323,共4页
Chinese Journal of Virology
基金
美国NIH基金(R03TW01192-04)
国家自然科学基金资助项目(30270069)
