皮肤软组织重复扩张的微观研究

Microscopic study on skin and soft tissue after repeated expansion

目的探讨皮肤软组织重复扩张的微观变化,为临床应用提供理论依据。方法以6头小型猪为实验动物,并分为正常对照组、常规扩张组、重复扩张组,分别切取皮肤标本,进行光镜结构和超微结构的观察,以及碱性成纤维细胞生长因子(bFGF)含量的测定。结果重复扩张的皮肤软组织肉眼观察基本健康,其微观结构在常规扩张的基础上有进一步的变化。胶原纤维损伤明显,细胞损伤较轻并且进一步增殖和功能更加活跃。皮肤中bFGF含量进一步增高。结论重复扩张过程中,皮肤软组织虽然有进一步损伤,但通过进一步加强生长和修复功能,仍然能保持基本健康,因此,重复扩张安全可行。

Objective The study was to evaluate the microscopic changes on skin and soft tissue after repeated expansion for clinical work. Methods Six little pigs were divideded as： conventional expansion group, repeated expansion group, and blank control group. Histologic, uhrastructure and bFGF of the skin were observed and measured in each group after samples had been made. Results The skin and soft tissue after repeated expansion were healthy on the whole. Compared with the conventional expansion group, there was more microscopic change in the repeated expansion group. Collagen fibers were injured evidently. Cells were injured slightly and proliferated much more, and moreover, they were more activated. The content of bFGF was more higher. Conclusions The skin and soft tissue after repeated expansion are healthy on the whole by more growth and more repair though repeated expansion may result in more injuries. So repeated expansion is safe and feasible.