侵袭性牙周炎龈沟液中硫化物的检测

Measurement of sulfides level in gingival crevicular fluid from aggressive periodontitis

目的:检测侵袭性牙周炎(AgP)龈沟液中硫化物浓度水平变化并探讨其与牙周临床指标的关系。方法:采用金刚牙周诊断仪进行龈沟液硫化物和牙周临床指标测定。选定实验组(T)16例侵袭性牙周炎病人,56个牙,共336个位点。其中健康牙(T1)16个,位点96个;炎症牙(T2)40个,位点240。对照组(C):全身、牙周健康者10例,20个牙,共120个位点。测定所选位点龈沟液(gingival crevicu lar flu id,GCF)中硫化物水平(su lcus su lph ide level,SUL),牙周袋探诊深度(pocket prob ing depth,PPD),牙周临床附着丧失水平(c lin i-cal attachm ent level,CAL),龈沟出血指数(su lcus b leed ing index,SB I)。结果:①3组受检牙龈沟液中硫化物浓度不同。侵袭性牙周炎炎症牙位组GCF中硫化物浓度(0.2210±0.0415)×10-6mol/L高于健康牙位组(P<0.05)、正常对照组;健康牙位组GCF中硫化物浓度(0.1025±0.0198)×10-6mol/L高于正常对照组(0.0523±0.0044)×10-6mol/L。②经相关性分析,侵袭性牙周炎炎症牙位组(T1)GCF中硫化物的浓度均值与PD、SB I和CAL均呈正相关关系,健康牙位组(T1)、正常对照组(C)GCF中硫化物浓度均值与PD、SB I及CAL无相关性。结论:侵袭性牙周炎病人龈沟液中的硫化物是参与牙周炎症反应的重要调节因子,其水平变化可反映牙周组织的炎症状态。

AIM ：To detect the concentration of sulfides in the gingival crevicular fluid （GCF） from aggressive periodontitis （AgP） and to evaluate the relationships between aggressive periodontitis and clinic periodontal parameters. METHODS： Sulfides level and periodontal clinic parameters were measured with Diamond Probe/Perio2000 System. The tested group （T） were 16 aggressive periodontitis subjects, 56 teeth and 336 positive sites, including healthy teeth （T1） 16, healthy positive sites 96 and inflammatory teeth （T2 ）40,240 positive sites. Control group （C） were 10 samples, 20 teeth, 120 positive sites. Sulcus sulphide level （SUL） in the gingival crevicular fluid, pocket probing depth （PPD）, clinical attachment level （CAL） and sulcus bleeding index （SBI） were measured. RESULTS： ①The sulfide concentrations of GCF among three groups Were significantly different. The sulfide concentration of GCF in inflamed group from aggressive periodontitis （AgP） was significantly higher than that in healthy group from aggressive periodontitis （AgP） （ P 〈 0.05 ） and healthy controll group （C） . The sulfide concentration of GCF in healthy group from aggressive periodontitis （AgP） was significantly higher than that in healthy control group. ② There were no correlations between sulfide concentration from healthy group of aggressive periodontitis （AgP） , healthy control group and clinic periodontal parameters, but there was significantly positive correlation between sulfide concentration in inflamed group from aggressive periodontitis （AgP） and bleeding on probing （ SBI）, pocket probing depth （PPD）. CONCLUSION： These results suggest that sulfides may play a role in pathogenesis of aggressive periodontitis. Its changes of concentration can reveal the periodontal tissue inflammatory conditions.