芦荟素抑制人表皮黑素细胞酪氨酸酶的最佳浓度选择

The optimum concentration of aloesin to inhibit tyrosinase activity of human epidermal melanocytes

目的:研究抑制人表皮黑素细胞酪氨酸酶的芦荟素最佳浓度。方法:选择不同浓度的中药单体芦荟素(分别为0.010、0.001、0.100、1.000和10.000mmol/L),作用于体外培养的人表皮黑素细胞,测定黑素细胞增殖率、酪氨酸酶活性和黑素含量。结果:10.000mmol/L芦荟素组导致黑素细胞死亡;与正常对照组相比,芦荟素浓度大于0.100mmol/L的黑素细胞增殖率明显下降(P<0.05);所有实验组黑素细胞的酪氨酸酶活性和黑素含量都显著降低(P<0.001)。结论:低浓度的芦荟素(0.001～0.010mmol/L)在不影响黑素细胞增殖的情况下可以显著抑制酪氨酸酶活性和黑素的合成。

Objective： To find the optimum concentration of aloesin to inhibit tyrosinase activity of human epidermal melanocytes. Methods： Different concentrations of aloesin, 0.001, 0.010, 0.100, 1.000, and 10.000 mmol/L respectively, were incubated with melanocytes. Proliferation rate, tyrosinase activity and melanin content of melanocytes were measured. Results： Aloesin induced the death of melanocytes in the high concentration （10.000 mmol/L）. Comparing with normal control, the proliferation rate of melanocytes became low （P〈 0.05） when the concentration of aloesin was more than 0.100 mmol/L, and tyrosinase activity and melanin content of melanocytes decreased dramatically （P〈 0.001） with all the concentrations mentioned above. Conclusion： A low and relatively safe concentration （0.001-0.010 mmol/L） is effective to inhibit the tyrosinase activity and melanogenesis other than the proliferation of human epidermal melanocytes.