羟基红花黄色素A对犬急性心肌缺血的保护作用

Protective effects of hydroxysafflor yellow A on acute myocardial ischemia in dogs

目的研究羟基红花黄色素A(HSYA)对犬急性心肌缺血的保护作用。方法采用结扎冠状动脉前降支的方法,造成犬急性实验性心肌缺血模型;超声多普勒方法测定心肌血流量;通过血氧分析研究心肌氧代谢;放射免疫法测定血浆内皮素(ET)的含量;用心外膜电图描记和定量组织化学染色方法计算心肌缺血程度、范围及梗死面积。结果HSYA可以增加心肌血流量以及冠状静脉窦内的血氧含量,并可以抑制ET从受损微小血管的释放,而且HSYA可以减弱心肌缺血的程度和范围,缩小缺血的面积。结论HSYA可以抑制ET的释放,增加心肌血流量,改善心肌氧代谢,抑制心肌缺血损伤。

Aim To study the protective effects of hydroxysafflor yellow A （HSYA） on acute myocardial ischemia in dogs. Methods The acute ischemia models were prepared by ligation of left anterior descending （LAD） artery. The myocardial blood flow （MBF） was determined by ultrasonic doppler, the oxygen metabolism was recorded using blood-oxygen analysis, and the ET was measured with radioimmunological assay. The experiments adopted epicardiogram mapping to measure the scope and the degree of myocardial ischemia, and used quantitative histological assay to determine the size of myocardial infarction. Results HSYA was showed to obviously increase the MBF and the oxygen volume in coronary vein sinus. And the treatment could inhibit the ET release from the injured microvessels. Furthermore, HSYA could alleviate the degree of myocardial ischemia （E-ST）, the scope of myocardial ischemia （N-ST）, and narrow the ischemic area.Conclusion HSYA was showed to inhibit the ET release, increase the MBF, improve the cardiac oxygen metabolism, and then inhibit the myocardial ischemia damage