老龄雌性大鼠胆碱能系统的氧化损伤及人参皂苷的保护作用

The damage of free radicals on cholinergic system of aged female rats and the protective function of ginsenosides

目的探讨自由基对基底前脑-海马/大脑皮质胆碱能系统的损害及人参皂苷(GS)的保护作用。方法W istar♀大鼠30只,随机分为3组:青年组、老龄组、给药组(第17mon龄始饲以GS至27mon龄)。采用紫外可见光光度计对3组大鼠基底前脑、海马结构和大脑皮质进行一氧化氮(NO)及丙二醛(MDA)含量测试;同时进行超氧化物歧化酶(SOD)活性测定。结果老龄组基底前脑、海马结构和大脑皮质NO及MDA含量均较青年组增高(P<0.01);给药组均较老龄组降低(P<0.01);相反,老龄组上述3个脑区SOD活性均比青年组降低(P<0.01),给药组均比老龄组增高(P<0.01)。结论GS具有增强基底前脑-皮质胆碱能系统抗氧化的能力,并能够提高SOD的活性。

Aim To investigate the damage of free radicals on cholinergic baso-cortex system and cortex and the protective function of ginsenosides （GS）. Methods 30 female Wistar rats were randomly divided into 3 groups （n = 10） ： young, aged and GS group （fed with GS from 18 th to 27 th month）. We tested nitric oxide （NO） and malondialdehyde （MDA） levels and superoxide dismutase（SOD） activity in basal forebrain, hippocampal format and cerebral cortex of 3 groups with spectrophotometer. Results The levels of NO and MDA in the three brain areas were higher in aged group （ P 〈 0. 01 ） but lower in GS group （ P 〈 0. 01 ）. The activity of SOD was lower in aged group （P〈0.01） but higher in GS group （P 〈0.01）. Conclusion Ginsenosides （GS） improved the antioxidative ability of cholinergic baso-cortex system.