摘要
目的:构建高通量的组织芯片,评价CA125、EPCAM联合和单一检测对卵巢癌诊断的价值,探讨免疫荧光染色及激光非共聚焦方法扫描组织芯片的可行性。方法:制备卵巢组织芯片,含82例样本,其中卵巢上皮性癌52例,正常卵巢组织30例。用免疫组织化学法检测CA125和EPCAM的表达,四格表法统计出四项诊断指标值。结果:联合CA125和EP-CAM,诊断卵巢癌的特异性由86·67%上升到93·33%,阳性预测值则升至96·97%。结论:免疫荧光技术具有特异性强、敏感性高、速度快等特点,用于组织芯片染色是可行的。CA125和EPCAM的联合检测可提高特异性和阳性预测值,对卵巢癌的诊断有较高价值。
Objective: To assess the value of CA12S and EPCAM for diagnosing and differentiating ovarian neoplasms and to evaluate the applicability of immunity fluorescence dyeing and unconfocal laser scanner by constructing and applying high-throughput tissue microarrays. Methods:The ovarian tissue chip was prepared, including 82 cases of formalin-fixed, paraffin-embedded tissue from 52 ovarian carcinomas and 30 normal ovarian tissues. CA12.5 and EPCAM were detected with immunohistochemical staining methods. The results were analyzed by referring to the pathology of the specimens to calculate the sensitivity, specificity, accuracy and expected positivity of the single monoclonial antibodies(MoAb) tests and the combinations of the tests. Results:The specificity of CA12.5 test was 86. 67%, the value would be increased to 93. 33% when the positive result of EPCAM was considered together, with the rate of expacted positivity being 96. 97%. Conclusion:The immunity fluorescence technique provide a reliable method to investigate marker expression by offering a sensitive, specific and rapid means. Joint detection of CA12.5 and EPCAM will increase the specificity of the immunosssay and expected positivity. The combination of CA12.5 and EPCAM is of high practical value for diagnosing ovarian cancer.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2006年第5期467-469,472,共4页
Chinese Journal of Immunology
关键词
卵巢癌
组织芯片
免疫组化
免疫荧光
Ovarian cancers
Tissue microarray
Immunohistochemistry
Immunity fluorescence