软骨组织工程中细胞外基质的研究

Extracellular matrix in cartilage tissue engineering

目的:分析软骨组织工程中软骨细胞、软骨基质、生长因子之间的相互作用及细胞外基质替代物的开发。资料来源:应用计算机检索Medline1995-01/2005-12有关软骨组织工程中软骨细胞外基质的文章,检索词为“cartilage;extracellularmatrix”,并限定文章语言种类为English。资料选择:对资料进行审阅,选取包括软骨组织工程的文章,并查阅全文。纳入标准:①软骨细胞外基质及其受体。②软骨组织重建相关生长因子。③软骨细胞外基质替代物。排除标准:Meta分析、综述文献、重复研究。资料提炼:共收集到1179篇关于组织工程软骨及细胞外基质的文献,纳入符合标准的文献29篇。资料综合:软骨组织无血管,其自我修复及重建能力有限,较小的创伤就会造成严重的软骨损伤及变性。软骨基质在软骨自身动态平衡及软骨修复等方面起着非常重要的作用,软骨细胞合成、分泌软骨基质并受软骨基质的调控。重建软骨组织就必须考虑软骨细胞及其周围环境的相互作用。结论:重建关节软骨基质、促进软骨复原,研究软骨细胞和其周围环境之间的相互作用是关键。

OBJECTIVE：To analyze the interaction among chondrocytes, extracellular matrix and growth factors, and the development of the substitute of extracellular matrix in cartilage tissue engineering. DATA SOURCES：A computer-based search of Medline database was undertaken to identify articles about extracellular matrix of cartilage published in English between January 1995 and December 2005 by using the key words of “cartilage;exttacellular matrix”. STUDY SELECTION：The literatures about cartilage tissue engineering were selected, and then the full texts were searched for. Inclusive criteria： ①The extracellular matrix of cartilage and its receptor. ②reconstroction of cartilage tissue related growth factors. ③The substitute of extracellular matrix in cartilage tissue engineering. Exclusive criteria：Reviews, repetitive Studies Or Meta analytical papers. DATA EXTRACTION：Totally 1179 articles about cartilage tissue engineering and extracellular matrix were collected, 36 accorded with the inclusive criteria. DATA SYNTHESIS：The cartilage itself lacks a blood supply to support repair and remodeling. Because of the limited capacity for spontaneous repair, minor injury to articular cartilage may lead to progressive damage and degeneration. The cartilage extracellular matrix （ECM） plays an important role in cartilage homeostasis and repair. Chondrocyte synthesizes and secretes ECM and is, in turn, regulated by ECM. Therefore, interaction botween the chondrocytes and their surroundings should be taken into account in reconstruction of cartilage tissue. CONCLUSION： Study on the interaction between chondrocytes and their surroundings is the key to reconstruct EMC and promote cartilage recovery.