塔玛亚历山大藻的生长研究

STUDY ON THE GROWTH OF ALEXANDRIUM TAMARENSE BALECH

在室内条件下研究了温度、N和P、维生素、抗生素对有毒赤潮甲藻塔玛亚历山大藻(香港株Ⅱ)生长的影响。结果表明,塔玛亚历山大藻的最适生长温度为21—25℃,最适N、P浓度分别为882—1765μmol/L和18—72μmol/L。复合维生素B1、B6、B12的加入有利于塔玛亚历山大藻的生长,而50U/mL以上的抗生素(氨苄青霉素液体)则对其有明显的抑制作用。

The present study was to determine the effects of water temperature, nitrogen and phosphorus concentrations, vitamin and antibiotic concentrations on the growth of toxic Alexandrium tanarense （ Hong Kong Chain Ⅱ） under experimental conditions. The strains of Alexandrium tamarense was isolated in 1998 from Hong Kong sea area where harmful algal blooms（HAB）had frequently occurred, some of which were highly toxic. The results showed that its optimum growth condition was at 21-25℃, 882-1765μmol N/L and 18-72μmol P/L respectively. When temperature was 21~C, nitrogen concentration was 1765μmol/L and phosphorus concentration was 72μmol/L, the specific growth rate calculated from microscopic cell counts over the exponential phase of growth attained the higher value of 0.21,0.28 and 0.28 d^-1 respectively. Incorporated vitamin made up of B1, B6 and B12 had positive effect on the growth of Alexandrium tamarense when the contents of them ranged from 0, 0 and 0μg/L to 500,500 and 1. Ottg/L. The dinoflagellate got their maximal growth rate O.26 d^-1 at the highest vitamin concentrations. While the antibiotic（liquid of ampicillin）exceeded 50U/mL had obviously inhibition on it. The cellule algae of Alexandrium tamarense was observed in the culture containing antibiotic, and the forming mechanism required extended studies.