经口给予黄芪提取物对H22肝癌荷瘤小鼠影响的实验研究

Empirical Studies on the Effect of Oral Administration of Astragalus membranaceus Extract to H22 Hepatocellular Carcinoma-Bearing Mice

目的:综合分析黄芪提取物对H22肝癌荷瘤小鼠肿瘤细胞增殖、凋亡及机体免疫功能的影响。方法:采用病理形态学观察、免疫组化、流式细胞术和免疫学方法研究灌胃不同剂量黄芪提取物(ASA)对H22荷瘤小鼠肿瘤细胞增殖与凋亡、脾脏CD4+/CD8+T淋巴细胞比例及腹腔巨噬细胞吞噬功能的影响,综合评价ASA对H22荷瘤小鼠的影响。结果:ASA对H22荷瘤小鼠肿瘤重量、肿瘤细胞增殖均无明显影响。除ASA0.031mg/g组外,ASA0.125mg/g、0.500mg/g和2.000mg/g组肿瘤细胞凋亡率和Bax的表达均明显高于对照组(P<0.01),而实验组Bcl-2表达则明显低于对照组(P<0.01)。ASA可增加荷瘤小鼠脾脏CD4+/CD8+比例,提高腹腔巨噬细胞吞噬能力。结论:ASA对H22肝癌荷瘤小鼠肿瘤细胞增殖无明显影响,但可在一定程度上促进肿瘤细胞凋亡,提高机体免疫功能。

Objective： To explore the comprehensive effect of Astragalus membranaceus extract （ASA） on the proliferation and apoptosis of H22 hepatocellular carcinoma cells and on the immune function of mice injected with H22. Methods： ASA was administered orally at doses of 0.031mg/g, 0.125mg/g, 0.5mg/g and 2mg/g, and the effects on growth and proliferation of H22 tumor cells in mice were evaluated by pathological observation, cellular PCNA immunohistochemical expression and flow cytometric proliferation index. Tumor cell apoptosis rate, expression of the apoptosis-related genes bcl-2 and bax, splenic CD4＋ T lymphocytes, CD8＋ T lymphocytes and the ratio of CD4＋/CD8＋ were quantitatively determined by flow cytometry. The phagocytotic function of macrophages was studied by observing peritoneal macrophages phagocytize chicken RBC. Results： After oral ASA administration at different dosages, no significant inhibiting effects on tumor growth or cellular proliferation were observed. Increased apoptosis rate, decreased Bcl-2 expression and increased Bax expression of H22 tumor cells in ASA treated groups could be found, but no significant correlation between ASA dose and these parameters were found. ASA administration could increase the splenic CD4＋ T lymphocyte fraction, thereby increasing the CD4＋/CD8＋ ratio, and increase peritoneal macrophage phagocytosis rates. Conclusion： Oral administration of ASA could not significantly affect the growth or cellular proliferation of H22 tumor cells, but it could to some extent increase the apoptosis rate of H22 tumor cells in vivo. It could also improve cellular immunity of the mice bearing H22.