传统热补针法对实验性关节炎家兔中枢镇痛机理的研究

Experimental study of central analgesia machanism on traditional acupuncture therapy of heat-reinforce for experimental arthritis rabbits

目的：观察传统热补针法对实验性关节炎家兔模型的镇痛效应和中枢镇痛机理。方法：将健康2—3月龄青紫蓝兔50只在测定痛阈并筛选后。按随机表分组法分为正常对照组、模型对照组、内服药物对照组、捻转针法对照组、热补针法治疗组5组，每组9只。以卵蛋白诱导关节炎疼痛造模，对正常对照组、模型对照组只是每天抓取、固定刺激。其余3组连续治疗6d后，观察各组家兔脑脊液中β-内非肤（β-EP）、八肤胆囊收缩素（CCK-8）、腰髓P物质（SP）的含量。结果：经治疗后，各治疗组家兔的痛阈均升高，有显著性意义（P〈0．01）；热补针法与药物和捻转针法之间无显著性差异（P〉0．05）。各治疗组家兔脑脊液中的镇痛物质均有不同程度的改变。其中热补组家兔脑脊液中的β-EP、CCK-8、SP明显升高（P〈0．05或P〈0．01），其作用优于内服药物组和捻转针法组（P〈0．01）。结论：传统热补针法有显著的中枢镇痛效应。

Objective：To observe analgesia effect and central analgesia mechanism of traditional acupuncture therapy of heat-reinforce to experimental arthritis rabbits. Methods：50 health rabbits were divided into 5 groups after determining pain threshold and selecting,and every group had 9 rabbits. Induced arthritis ache model with egg albumin. Rabbits of control group and model group were grahed and irritated every day, the rest were treated continuously. 6 days latter, compared the change state of pain threshold and the content of β-EP,CCK-8 and SP in rabbits＇ CSF. Results：After treating, pain threshold of rabbits in each treating groups rised. The content of β-EP, CCK-8 and SP in CSF changed prominently,and acupuncture therapy of heat-reinforce could increased prominently the level of β-EP, CCK-8 and SP （P 〈0.05 or P 〈 0.01 ）. Conclusion： Traditional acupuncture therapy of heat-reinforce has prominent effect of central analgesia.