摘要
使用荧光光谱和紫外光谱研究Cu(Ⅱ),N i(Ⅱ)存在的条件下对盐酸小檗碱(berberine chloride d i-hydrate,BC)与牛血清白蛋白(BSA)相互作用的影响。得到的猝灭常数KSV表明,金属离子Cu(Ⅱ)或N i(Ⅱ)的存在不影响其猝灭机理。基于F rster非辐射能量转移机理,获得了盐酸小檗碱与牛血清白蛋白的212位色氨酸残基间的距离。讨论了金属离子与BSA的结合构型对BC与BSA相互作用的影响。
The interaction between berberine chloride dihydrate (BC) and bovine serum albumin (BSA) in the presence of Cu ( Ⅱ ) or Ni ( Ⅱ ) using fluorescence and UV absorption spectrometric methods was studied. The quenching constant obtained indicated that the presence of Cu ( Ⅱ ) or Ni ( Ⅱ ) did not affect the quenching mechanism. The binding distance R between BC and W^212 of BSA was calculated according to the non-radiation energy transfer theory of Forster. The effects of the configuration between the metal ions and BSA on the interaction of BC with BSA were discussed.
出处
《分析测试学报》
CAS
CSCD
北大核心
2006年第4期48-51,共4页
Journal of Instrumental Analysis
关键词
牛血清白蛋白
盐酸小檗碱
荧光法
铜离子
镍离子
Bovine serum albumin
Berberine chloride dihydrate
Fluorescence
Cu( Ⅱ )
Ni(Ⅱ )