Cu(Ⅱ),Ni(Ⅱ)存在条件下盐酸小檗碱与牛血清白蛋白相互作用的研究

Study on the Interaction between Berberine Chloride Dihydrate and Bovine Serum Albumin in the Presence of Cu(Ⅱ) or Ni(Ⅱ)

使用荧光光谱和紫外光谱研究Cu(Ⅱ),N i(Ⅱ)存在的条件下对盐酸小檗碱(berberine chloride d i-hydrate,BC)与牛血清白蛋白(BSA)相互作用的影响。得到的猝灭常数KSV表明,金属离子Cu(Ⅱ)或N i(Ⅱ)的存在不影响其猝灭机理。基于F rster非辐射能量转移机理,获得了盐酸小檗碱与牛血清白蛋白的212位色氨酸残基间的距离。讨论了金属离子与BSA的结合构型对BC与BSA相互作用的影响。

The interaction between berberine chloride dihydrate （BC） and bovine serum albumin （BSA） in the presence of Cu （ Ⅱ ） or Ni （ Ⅱ ） using fluorescence and UV absorption spectrometric methods was studied. The quenching constant obtained indicated that the presence of Cu （ Ⅱ ） or Ni （ Ⅱ ） did not affect the quenching mechanism. The binding distance R between BC and W^212 of BSA was calculated according to the non-radiation energy transfer theory of Forster. The effects of the configuration between the metal ions and BSA on the interaction of BC with BSA were discussed.