摘要
首次将绍兴黄酒中的肽类组分进行大孔吸附树脂柱层析和高效凝胶过滤色谱以及反相色谱的多步提取纯化与抑制血管紧张素转换酶(ACE)活性试验,并首次利用基体辅助激光解吸电离-飞行时间串联质谱分析和液相色谱-电喷雾电离四极杆-飞行时间串联质谱联用分析鉴定出黄酒中4种ACE活性抑制肽的氨基酸序列为:VEDGGV、PST、NT和LY。
Inhibitory peptides for angiotensin converting enzyme（ACE） were isolated from Chinese rice wine by hollow fibre ultrafiltration followed by column chromatography using AB-8 macroporous adsorption resins. Fractions of the peptides were purified on the TSK gel 2000 SWXL column using high performance gel filtration chromatography and on the symmetry C18 column using semi-preparative reversephase high performance liquid chromatography, respectively. Their ACE inhibitory activities were then determined. Amino acid sequences of four bioactive peptides identified by MALDI-TOF-TOF-MS and LC -ESI-Q-TOF-MS were VEDGGV, PST, NT and LY, respectively.
出处
《分析测试学报》
CAS
CSCD
北大核心
2006年第4期74-77,共4页
Journal of Instrumental Analysis
基金
浙江省经贸委2002年第一批省技术创新项目
2003年绍兴市第一批科技计划项目(工业重点项目
2003106)
关键词
黄酒
血管紧张素转换酶
活性肽
氨基酸序列
高效液相色谱
基体辅助激光解吸电离
串联飞行时间质谱
电喷雾电离
四极杆飞行时间串联质谱
Chinese rice wine
Angiotensin converting enzyme
Bioactive peptides
Amino acid sequences
High performance liquid chromatography
Matrix-assisted laser desorption/ionization
Timeof-flight mass spectrometry
Electrospray ionization
Quadrupole-time of flight mass spectrometry
