反相HPLC法同时测定烟草中的6种有机酸

Simultaneous Determination of Six Organic Acids in Tobacco with Reverse-phase High Performance Liquid Chromatography

建立了用反相高效液相色谱法同时快速测定烟草中柠檬酸、苹果酸和草酸等6种有机酸的方法。采用的色谱柱为Hypersil C18（250mm×4．6mm，5μm），流动相0.01mol／L NaH2PO4（pH2．98）和无水甲醇（体积比为98：2），流速为1．0mL/min，柱温25℃，检测波长210nm。有机酸的回收率为98．2％-104.3％；相对标准偏差为0．61％-3．38％；检出限0．04—0．53μg／mL，分析时间不超过7min。并分析了4种转光地膜和普通地膜覆盖的K326初烤烟叶中这6种有机酸的含量。

A method for simultaneous and rapid determination of 6 organic acids （including citric, malic and oxalic acid） in tobacco with reverse-phase high performance liquid chromatography （HPLC） was developed. HPLC was performed on a Hypersil C18 column （250mm × 4.6mm, 5μm） at 25℃, using 2% CH3OH- 98%NaH2PO4（0.01mol/L; pH2.98） as mobile phase at the flow rate of 1.0mL/min and the detection wavelength of 210nm. The recoveries of the organic acids ranged from 98.2 % to 104.3 % with relative standard deviations of 0.61% - 3.38 %, and the limits of detection were 0.04 - 0.53μg/mL （S/N = 3 ）. The runtime was less than 7min. The 6 organic acids in cured tobacco leaves of K326 covered with four sun light-conversion films and one ordinary film were also analyzed.