摘要
报道了一种新的DNA晶体的生长体系,即甲醇-水(甲醇体积比50%)体系.甲醇-水体系是DNA晶体生长较宽松、容易的环境.TEM和AFM观察到在甲醇-水体系中生长的DNA晶体比在水中生长的DNA晶体更大,而且DNA晶体的构象发生了明显的变化,可能存在有B-,A-和Z-3种构象.
An approach for the growth of larger sizes of DNA lattices in a methanol-water solution is described. Two types of lattices have been obtained. We suggest that the DNA lattices formed in methanol/water are in a meta-stable form. Different from those grown in aqueous buffer, where the lattice is formed only with B-DNAs, the lattice should be formed by B-, A- and Z-DNAs.
出处
《江西师范大学学报(自然科学版)》
CAS
北大核心
2006年第3期291-293,299,共4页
Journal of Jiangxi Normal University(Natural Science Edition)
基金
国家"863"计划(2004AA302G12)资助项目
关键词
DNA晶体
甲醇-水体系
构象
DNA lattices
methanol-water solution
formation