多元校正—吸光光度法同时测定药物中的甲硝唑和芬布芬及酮洛芬

Simul Taneous Spectrophotometric Determination of Metronidazole,Fenbufen and Ketoprofen Using Multivariate Calibration Method

甲硝唑、芬布芬和酮洛芬在紫外区均有吸收，但吸收光谱相互重叠而形成干扰，难以进行直接分析。在pH=7．0的KH2PO4-Na2HPO4缓冲溶液中对甲硝唑、芬布芬和酮洛芬三组分混合溶液进行光度测定，所得的重叠吸收波谱数据用化学计量学多元校正方法处理，建立相应的数学校正模型，进而用于未知溶液的浓度预报分析。该法还被用于实际药物样品的测定，获得了较好的定量分析结果。本实验中甲硝唑、芬布芬和酮洛芬的线性范围分别为1.0-10.0μg／mL，0．2-6．0μg／mL和1．0—16．0μg／mL；检出限分别为0．34μg／mL，0．08μg／mL和0．32μg／mL。

Metronidazole Fenbufen and Ketoprofen have absorption property in ultraviolet zone, and their absorption spectra are overlapping. Generally,they can not be determined individually by spectrophotometry without prior separation. In this paper, the chemometrics multivariate calibration method was applied to the simultaneous determination of these three compounds in a potassium dihydrogen phosphate - sodium hydrogen phosphate buffer solution （ pH7. 0） ,and the analytical results was compared with classical least squares （CLS）, principal components regression （PCR） and partial least squares （PLS）. The linear ranges of metronidazole fenbufen and ketoprofen are 0.8 - 10. 0,0.2 -6.0 and 1.0 -16.0 μg/mL,respectively. The proposed method was successfully applied to the analysis of some medicine products with satisfied results.