牛GV期卵母细胞冷冻保存后发育潜力的研究

Research on the Development Competence of Frozen-Thawed Bovine GV Oocytes

二甲基亚砜(DMSO)、丙二醇(PROH)、乙二醇(EG)和甘油(GL)4种冷冻保护剂程序化冷冻牛GV期卵母细胞的结果表明,EG和PROH的保护效果比GL和DMSO好。4种不同冷冻方法冷冻保存牛GV期卵母细胞,比较解冻后卵母细胞的体外成熟率、受精后卵裂率。结果表明,在程序化冷冻法与细管玻璃化法(Straw)之间的差异不显著(P>0.05),在开放式拉管法(OPS)与毛细玻管法(GMP)之间的差异不显著(P>0.05);但OPS和GMP与程序化冷冻法和Straw之间的差异极显著(P<0.01)。玻璃化冷冻效果优于程序化冷冻。说明GMP和OPS玻璃化冷冻优于Straw玻璃化冷冻。说明可以采用GMP方法冷冻保存牛GV期卵母细胞。

Using the programmed freezing method to freeze the bovine ooeytes with 1.5 mol/L DMSO, PROH,EG and GL, the effects of EG and PROH were better than GL,DMSO. Using programmed freezing method, Straw vitrification, open-pulled-straw（OPS）, glass mieropipette（GMP） to freeze the bovine GV ooeytes, frozen-warmed ooeytes were matrured and fertilized in vitro. The cleavage ratio of programmed freezing method and Straw vitrification was not significantly different（ P 〉0.05）. The cleavage ratio of OPS and GMP was similar, and the results was not significantly different（ P〉0.05） ,but was significantly different with programmed freezing method and Straw vitrifieation（ P〈0.01）. This demonstrated that vitrification was better than programmed freezing method,GMP and OPS was better than Straw vitrifieation, that the effect of GMP and OPS was similar. The conclusions proved that GMP vitrification procedure can be used to eyro-preserve bovine GV ooeytes.