桃果肉颜色、果皮茸毛和花粉育性性状的分子标记

Molecular Markers Linked to Specific Characteristics of Prunus persica(L.) Batsch

以91-42-51×瑞光2号的杂交后代共48个单株为试材,采用集群分离分析法(BSA),对桃果实的有毛/无毛、白肉/黄肉以及无花粉/有花粉3个性状进行了分子标记研究。研究获得SSR标记UDP96-018(300bp)与有毛性状连锁,遗传距离4·5cM,SSR标记UDP98-407(680bp)与白肉性状连锁,遗传距离为2·2cM;根据拟南芥雄性不育序列标记设计的引物扩增出的两个片段NNJ-I(600bp)和NNJ-I(900bp)与桃无花粉性状之间的遗传距离为0cM。这些标记的获得为桃分子标记辅助育种提供了有效的筛选手段。

A population of 48 individuals was developed from cross ‘91-42-51＇ בRuiguang 2＇ , and was used to find molecular markers linked to characters as peach or nectarine, yellow or white flesh, male sterility or male fertility by bulked segregant analysis （BSA）. The results showed that SSR marker UDP96-O18 （300 bp） was found linked to peach gene with a distance of 4. 5 cM, and UDP98-407 （680 bp） linked to white flesh gene with a distance of 2.2 cM. Marker NNJ-I （600 bp） and NNJ-I （900 bp） amplified by primers which were designed according to male sterility sequence of Arabidopsis thaliana were tightly linked to male sterility gene with a distance of 0 cM. Markers linked to these characters obtained in this study would provide effective tools for marker assisted selection in peach breeding.