番茄乙烯受体基因反义表达对果实成熟的影响

Effect of Ethylene Receptor Genes Antisense Transformation on Fruit Ripening

以乙烯受体基因LeETR1和LeETR2的转反义基因株系ale1和ale2为材料,对果实成熟特性进行了研究.转基因番茄果实与对照相比,呼吸速率下降,但乙烯释放速率增加1～4倍,呼吸高峰和乙烯跃变高峰出现的时间没有改变,表明果实成熟起始时间没有改变.ale1果实成熟后期着色速度减慢,红熟果色泽a值、番茄红素和类胡萝卜素含量都显著低于对照;但果实多聚半乳糖醛酸酶活性上升和硬度下降速度都显著高于对照.这说明LeETR1基因与果实成熟品质特征的形成可能存在比较复杂的关系.

Fruit ripening characteristics of antisense transgenic lines alel and ale2 respectively from ethylene receptor genes LeETR1 and LeETR2 were studied. Compared to the wild type, transgenic lines displayed that respiration rate decreased, whereas ethylene production increased 1 -4 folds, which suggest that starting time of fruit ripening were not affected. At later stage of alel fruit ripening, a-value, carotenoid and lycopene contents was lower than that in wild type fruit. Although the speed of fruit coloring slow down in alel, the polygalacturonase increasing and firmness decreasing were sped up. The results suggest that there is some intricate relation between LeETR1 and fruit ripening.