利用cDNA-AFLP检测甘蓝雄性不育相关基因的时序性表达

Sequential Expression of Fertility-related Genes Detected by cDNA-AFLP in Different Types of Cabbage Male Sterile Lines

通过cDNA-AFLP技术分别对4种具有不同败育时期特征的甘蓝雄性不育材料和遗传背景一致的可育材料进行分析,揭示了4种不育类型育性相关基因的时序性表达特征,并对4种育性相关基因分别选取1个转录表达片段(TDFs)克隆测序。结果表明,获得的113条不同表达方式TDFs可分为12种模式:其中按照不同雄性不育败育顺序表达中断的有A、B、C、D4种模式,分别代表造孢细胞期、花粉母细胞期、四分体前期和四分体后期发育中断的育性相关基因。这4种表达模式包括76条TDFs,占总TDFs的67·25%。结合发育中断模型和序列分析结果,推测糖基水解酶家族基因、具有VQ结构域的基因、富含甘氨酸蛋白和促进游离小孢子解离的果胶裂解酶基因可能分别参与上述花药发育4个时期雄性育性的建成。

Elucidating the gene expression in anther development is one of the key steps to develop new types of male sterility. Four types of male sterile cabbage lines and their corresponding fertile lines were used to analyze the gene expression by cDNA-AFLP. In total, we got 113 differentially expressed TDFs, which were classified into 12 groups. Among them, 76 TDFs （67.25% of the total） were classified into group A, B, C and D, which were sequentially interrupted according to the different sterile stages in four male sterile lines. Sequence analysis of representative TDFs from each group showed that glycosyl hydrolase family 1 protein, VQ motif-containing protein, glycine-rich protein and pectate lyase family protein possibly involved in sporogenesis cell, pollen mother cell, pre-tetrad and post-tetrad stage male development respectively.