刚竹属(Phyllostachys)23个观赏竹种间亲缘关系的RAPD分析

RAPD Analysis of Genetic Relationships among the 23 Ornamental Bamboo Species in Phyllostachys

本文采用RAPD技术分析了刚竹属23个种或变种的DNA多态性.从37个随机引物中选出多态性效果好的6个引物,共获得47个DNA扩增片段,大小处于0.3～1.05kb,扩增表现出明显的多态性,高达93.6%.对RAPD产物进行相似系数统计和聚类分析,结果同形态分类结果基本吻合,但其中个别竹种出现了不同结果,即：与种和其变种是聚在一类的结论不一致,因此,对个别竹种有待进一步研究.

23 species of Phyllostachys were analyzed by amplified polymorphic DNAs （RAPDs）. Six primers out of 27 random primers showed high polymorphism. Totally 47 of the polymorphic amplification DNA fragments were obtained. The length ranged from 0. 3 kb to 1.05 kb. The polymorphic amplification has showed distinct polymorphism, about 93.9%. The results of cluster analysis and similarity coefficient statistics from RAPD, analysis were dentical in the main with that from morphological taxonomy, although a few species not in good agreement with the morphological properties. This species need to be studied further.